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Single-molecule trajectory analysis for unraveling receptor–b-arrestin interactions| title | Single-molecule trajectory analysis for unraveling receptor–b-arrestin interactions |
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| start_date | 2024/07/12 |
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| schedule | 11h30 |
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| online | no |
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| location_info | / |
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| summary | At the mesoscopic scale of cellular processes, the passive motion of proteins and receptors is dominated by diffusion. While diffusion is a rather inefficient transport mechanism, cells through evolution have found strategies to improve molecular reaction rates. These strategies rely on the interplay between protein structures and cell organization to exploit diffusion mechanisms. Measuring diffusion properties and their variations in space and time allows one to understand microscopic mechanisms of dynamical cellular processes and their interplay with cell organization.
We will discuss how fluorescence microscopy techniques and single-molecule trajectory analysis can be combined to understand some aspects of signal transduction [1]. Despite recent structural advances, the mechanisms that govern interactions of membrane-bound receptors with intracellular arrestin molecules at the plasma membrane remain elusive. We dissect the complex sequence of events involved in b-arrestin interactions with both receptors and the lipid bilayer. We carefully characterize the spatiotemporal co-dynamics of receptors and arrestins. We show that receptor and arrestin diffusion have multiple states linked to biological function.
Unexpectedly, our results reveal that b-arrestin spontaneously inserts into the lipid bilayer and transiently interacts with receptors via lateral diffusion on the plasma membrane. Moreover, they indicate that following receptor interaction, the plasma membrane stabilizes b-arrestin in a longer-lived membrane-bound state, allowing it to diffuse to clathrin-coated pits separately from the activating receptor.
[1] Grimes J, Koszegi Z, Lanoiselée Y, et al. Plasma membrane preassociation drives β-arrestin coupling to receptors and activation. Cell. 2023;186(10):2238-2255.e20. |
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| responsibles | NC |
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Workflow history| from state (1) | to state | comment | date |
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| submitted | published | | 2024/07/04 13:56 UTC |
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