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Building a bridge between in vivo and in vitro experiments with computer models: modelling the bursting activity in rat supraoptic cells| old_uid | 124 |
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| title | Building a bridge between in vivo and in vitro experiments with computer models: modelling the bursting activity in rat supraoptic cells |
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| start_date | 2005/11/02 |
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| schedule | 11h30 |
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| online | no |
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| details | Invité par Françoise Moos Inra 1244 |
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| summary | Building a bridge between in vivo and in vitro experiments with computer models: modelling the bursting activity in rat supraoptic cells.
In vivo, the milk ejection reflex in the rat is associated with the emission of short bursts of very high frequency action potentials by oxytocin secreting cells in the hypothalamus. These bursts are highly synchronized across the entire population of cells. In contrast, when the rat is not lactating, oxytocin cells fire a slow, Poisson distributed spike train that has no correlationn across the population.
It has been extremely difficult to replicate this phenomena in vitro, and so as yet we have little understanding of the ionic mechanisms that are the substrate for the synchronized bursting.
We have built a computer model for an oxytocin cell that incorporates both in vitro electrophysiological data, and also data from our calcium imaging studies. First we show that this model replicates all of the experimental protocols commonly used in our experimental studies. |
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| responsibles | Renaud, Deris |
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