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Axonal and dendritic growth in CNS slice cultures| old_uid | 851 |
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| title | Axonal and dendritic growth in CNS slice cultures |
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| start_date | 2006/03/15 |
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| schedule | 11h30 |
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| online | no |
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| location_info | Bat B, 5e étage, salle de conférence |
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| details | Séminaire supplémentaire |
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| summary | The outgrowth of axons and dendrites is a fundamental process for the development of a neuron because it will define its synaptic connections within the neural network. A
better understanding of the molecules and mechanisms which regulate and control axonal and dendritic growth during development and in the mature nervous system will not only deepen our understanding of the nervous system but is also required for improving interventional strategies aiming at better CNS repair after lesions or in nervous system diseases. In our laboratory we are employing tissue culture model systems which allow the study of axonal and dendritic growth within the CNS microenvironment, thereby reducing the need for animal experiments. In the first part of the presentation a novel in vitro model system of spinal cord injury will be presented using longitudinal sagittal slice cultures of mouse spinal cord. Initial studies of regeneration of intrinsic spinal cord axons in this culture system reveal a surprising potential of these fibers to regenerate within the spinal cord. The second part of the presentation will focus on the regulation of dendritic growth and development of cerebellar Purkinje cells. In previous work we have shown that activity of Protein Kinase C is an important regulator of Purkinje cell dendritic development. In contrast, the blockade of glutamate receptor mediated neurotransmission or the absence of signaling by the neurotrophin BDNF have little effect on Purkinje cell dendritic arbors. It is well known that in cerebellar long term depression PKC is activated by metabotropic
glutamate receptor (mGluR) stimulation. In more recent experiments we have explored
whether activation of mGluRs would also affect Purkinje cell dendritic development. Initial findings will be presented suggesting that activation of mGluRs does inhibit Purkinje cell dendritic development through a signalling pathway not involving PKC activation. |
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| responsibles | Doricchi |
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