|
Astroglial exocytosis: a note of caution from the perspective of a single-vesicle spectrosopist| old_uid | 1646 |
|---|
| title | Astroglial exocytosis: a note of caution from the perspective of a single-vesicle spectrosopist |
|---|
| start_date | 2006/10/23 |
|---|
| schedule | 11h30 |
|---|
| online | no |
|---|
| details | Invité par JP Mothet CRI /INB |
|---|
| summary | Glial cells have recently been implicated in bidirectional signaling from and to neurons at central synapses. Equiped with a plethora of neurotransmitter receptors and releasing neuroactive substances that classically have been considered as transmitters and neuromodulators of only neuronal origin, astroglia are increasingly being placed in the concept of a 'tripartite synapse' that -in addition to the classically recognized pre- and postsynaptic compartments-- involves glial processes that actively stimulate neuronal activity. Among the different pathways suggested for a regulated 'gliotransmitter' release, our lab has focused on the Ca(2+)-regulated vesicular (exocytotic) release of neuroactive substances by cortical astroglia. Using a combination of molecular genetics, single-vesicle imaging and spectrocopic techniques, we tagged, tracked and classifed astroglial vesicular compartments and their response to various challenges. Our data contradicts earlier observations of a large abundance of glutamatergic near-membrane vesicles in cultured astroglia and calls for a reconsideration of co-localization data based on EGFP expression and Acridine Orange labelling. Instead, we emphasize the importance of a highly dynamic population of secretory lysosomes. |
|---|
| responsibles | Deris |
|---|
| |
|