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Fast calcium imaging of Purkinje cells: a window on dendritic function| old_uid | 7782 |
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| title | Fast calcium imaging of Purkinje cells: a window on dendritic function |
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| start_date | 2009/12/07 |
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| schedule | 16h |
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| online | no |
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| summary | Implementation of local and global calcium signaling is a major function of dendrites. Dendritic calcium electrogenesis is the result of complex interactions between synaptic inputs, dendritic excitability and dendritic morphology. These interactions fix the rules for the induction of Ca-dependent plasticity and ultimately control information processing and information storage in neuronal networks. We have recently developed a fast digital scanning mode, based on the use of acousto-optic deflectors, that allows high frame rate multipoint optical recordings. Using this so-called random-access multiphoton microscopy, we have monitored calcium influx in Purkinje cell dendrites at high temporal resolution (5kHz) following stimulation of the parallel fiber and climbing fiber inputs. We show that the genesis of dendritic calcium spikes in Purkinje cells is a regulated process and that different calcium channels mediate subthreshold and suprathreshold calcium signaling. We will discuss the significance of these findings for the induction of plasticity and learning at parallel fibers to Purkinje cell synapses. |
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| responsibles | Levenes, Mongillo |
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