In vivo optical imaging of brain energy metabolism

old_uid12594
titleIn vivo optical imaging of brain energy metabolism
start_date2016/11/25
schedule11h30
onlineno
location_infoBât. 2, 2e étage
detailsInvitant : Valentin Nagerl, Team leader: Synaptic Plasticity and Superresolution Microscopy
summaryThe lack of adequate methods to investigate brain energy metabolism with the required spatio-temporal resolution in the intact organism has hampered significant advances in the field. Förster resonance energy transfer (FRET) sensors specific for energy substrates, such as glucose, lactate and pyruvate have been developed and successfully used in cultured cells and in brain slices. A major advantage of these FRET sensors is that they do not interfere with the intrinsic metabolite concentrations and pathways. In addition to unsurpassed spatial resolution, FRET microscopy can also detect fast metabolic dynamics. Furthermore, these sensors have great potential for in vivo studies in combination with two-photon microscopy.
responsiblesDeris