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Three-dimensional Ca2+ imaging of astrocytes and astrocyte-synapse interactions| old_uid | 14138 |
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| title | Three-dimensional Ca2+ imaging of astrocytes and astrocyte-synapse interactions |
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| start_date | 2017/06/23 |
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| schedule | 11h30 |
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| online | no |
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| summary | Astrocyte communication is typically studied by two-dimensional Ca2+ imaging, but this method has not yielded conclusive data on the role of astrocytes in synaptic and vascular function. We developed a three-dimensional two-photon imaging approach and studied Ca2+ dynamics in entire astrocyte volumes, including during axon-astrocyte interactions. Results indicate that Ca2+ activity in an individual astrocyte is scattered throughout the cell, largely compartmented between regions, preponderantly local within regions, and heterogeneously distributed regionally and locally. Processes and end-feet display frequent fast activity, whereas the soma is infrequently active. Results also indicate that astrocytes respond locally to minimal axonal firing with time-correlated Ca2+ spots |
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| responsibles | Bousquet |
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