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New optogenetic tools to visualize and control neural activity| old_uid | 14449 |
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| title | New optogenetic tools to visualize and control neural activity |
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| start_date | 2017/10/13 |
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| schedule | 11h30 |
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| online | no |
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| summary | Molecular engineering of improved fluorescent proteins (FPs) and innovative FP-based indicators has been a major driving force behind advances in cell biology and neuroscience for the past two decades. Among these tools, FP-based indicators (i.e., FP-containing proteins that change their fluorescence intensity or color in response to a biochemical change) have uniquely revolutionized the ability of biologists to visualize the otherwise invisible world of intracellular biochemistry. Similarly, light-activated protein-based actuators now enable researchers to control cellular activities with precise spatial and temporal resolution. In this seminar I will describe our most recent efforts to use protein engineering to make a new generation of versatile FP-based tools optimized for in vivo imaging and manipulation of cellular activity. Specifically, I will present our efforts to convert red and near-infrared FPs into indicators for calcium ion, membrane potential, and neurotransmitters, and describe our recent efforts to exploit FPs for optical control of protein activity and gene expression. |
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| responsibles | Bousquet |
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