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Low-birth-weight rats caused by mild intrauterine hypoperfusion reproduce neurodevelopmental disorders, by Masahiro TSUJI (MD, PhD). Department of Food and Nutrition, Kyoto Women’s University, Japan| old_uid | 17763 |
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| title | Low-birth-weight rats caused by mild intrauterine hypoperfusion reproduce neurodevelopmental disorders, by Masahiro TSUJI (MD, PhD). Department of Food and Nutrition, Kyoto Women’s University, Japan |
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| start_date | 2019/09/05 |
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| schedule | 14h-16h |
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| online | no |
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| summary | Severe intrauterine ischemia is detrimental to developing fetuses, especially to their brains. However, the influence of mild intrauterine hypoperfusion (MIUH) on the developing brain is not well understood; because optimal animal models are still lacking. Recently, we developed a novel rat model of MIUH, which results in fetal growth restriction (FGR) and low-birth-weight (LBW). The objective of the present study was to evaluate the influences of MIUH on neurological and behavioral development and to discuss putative mechanisms.
We induced MIUH via arterial stenosis with metal microcoils wrapped around the bilateral uterine and ovarian arteries in pregnant rats on embryonic day 17 (E17), which is considered to be equivalent to embryonic weeks 20-25 in humans. Microcoil stenosis decreased blood flow to the fetuses and placentas to 84% and 72% of the pre-stenosis level, respectively. Fetuses were delivered via spontaneous labor and LBW (≤5.5g) pups were used. Pups underwent behavioral tests for executive functions. Low molecular compounds in brain slices were evaluated by MALDI-mass spectrometry imaging. Metabolites in the brains were quantified via metabolomics analysis. |
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| responsibles | Sarlegna |
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