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Subtype-specific neuron to microglia communication of the neocortex| old_uid | 18849 |
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| title | Subtype-specific neuron to microglia communication of the neocortex |
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| start_date | 2021/03/15 |
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| schedule | 16h |
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| online | no |
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| details | Invited by: Alla SOLOD & Robin DARD
Participer à la réunion Zoom
https://zoom.us/j/97352910495?pwd=V3hkdmowdkxOV1dhYVhQUjJFY1dkZz09
ID de réunio : 973 5291 0495
Code: QQb4Ur |
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| summary | Microglia are the tissue resident macrophages of the brain that play critical roles in neural development and neuronal circuit function in health and disease. These brain-locked cells exist in multiple transcriptional states, yet it is poorly understood how these states are established, and whether they are influenced by their cellular environment. Furthermore, there is a lack of understanding of the bidirectional, cellular communication between neurons and microglia under homeostatic conditions. In our most recent work, we combine immunohistochemistry, single cell sequencing technology and advanced bioinformatic analyses to uncover the nuanced interaction between pyramidal neuron class identity and microglia of the cerebral cortex layers. We find that microglia density and state identity are instructed by the composition of local pyramidal neuron subtypes. By selectively altering the identity or laminar distribution of pyramidal neurons, we examine a profound and coordinated shift in the density and transcriptional status of local, layer-specific microglia. We then construct a neuroimmune ligand-receptor atlas between individual classes of excitatory neurons and microglial states by harnessing their single cell transcriptomes. This bioinformatic ligand-receptor interactome unmasks the rules of neuron-to-microglia communication including interactions driven by neuronal class identity or communication shared among all excitatory neurons and microglia. Our findings highlight the fundamental role that neuronal identity plays in locally controlling microglia and uncover previously unappreciated nuance in neuron-microglia interaction. |
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| responsibles | Bernier |
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