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Constructing and deconstructing centrioles| title | Constructing and deconstructing centrioles |
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| start_date | 2023/03/23 |
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| schedule | 12h |
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| online | no |
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| location_info | auditorium LBD - C7 |
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| summary | Understanding the organizing principles driving assembly and disassembly of cellular organelles is an important pursuit in biology. The centriole is a microtubule-based organelle that is essential for the formation of flagella, cilia and centrosomes.
The centriole has a signature 9-fold radially symmetrical arrangement of microtubules and is organized around a likewise symmetrical cartwheel that is essential for the onset of organelle biogenesis. The cartwheel comprises a stack of 9-fold radially symmetrical rings containing the protein SAS-6.
During my seminar, I will discuss how we deployed a novel high-speed atomic force microscopy (AFM) modality to reconstitute and monitor SAS-6 assembly dynamics with utmost spatial and temporal resolution.
Moreover, I will show how we have used Ultrastructure Expansion Stimulated Emission Depletion microscopy (U-Ex STED) to unravel the distribution of C. elegans centriolar proteins with great precision. Finally, I will discuss work in which we capitalize on the stereotypical cell lineage of C. elegans to investigate centriole fate in a comprehensive manner in a developing organism. |
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| responsibles | Carré, Hervé |
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Workflow history| from state (1) | to state | comment | date |
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| submitted | published | | 2023/03/16 15:27 UTC |
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