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Holographic manipulation of neuronal circuits in head fixed and freely moving mice| title | Holographic manipulation of neuronal circuits in head fixed and freely moving mice |
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| start_date | 2026/05/26 |
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| schedule | 11h |
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| online | no |
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| location_info | salle de conférence |
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| summary | The genetic targeting of neuronal cells with activity reporters, such as calcium or voltage indicators, has driven a paradigmatic shift in neuroscience, where photons have replaced electrons in reading large-scale brain activities at cellular resolution. Simultaneously, optogenetics has shown that targeting neuronal cells with photosensitive microbial opsins enables the transduction of photons into electrical currents of opposing polarities. This allows for the activation or inhibition of neuronal signals in a minimally invasive manner.
These advances have, in turn, spurred the development of sophisticated wavefront-shaping techniques to enable "all-optical" interrogation of deep brain circuits with high spatial and temporal resolution across large volumes1.
In this presentation, we will discuss the most recent approaches that we have recently proposed to enhance the capacity for patterned all-optical circuit manipulation. These approaches enable efficient in vivo two-photon and three photon multitarget optogenetic photostimulation2 and two-photon voltage imaging3,4 in both head-fixed and freely moving mice5,6. As an example of patterned optogenetics, we will present a recent experiment demonstrating high-throughput connectivity mapping in the mouse visual cortex5. |
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| responsibles | Burban |
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Workflow history| from state (1) | to state | comment | date |
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| submitted | published | | 2026/05/27 07:20 UTC |
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