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Genomic distancing in yeast: the control of conflicts between DNA-associated machineries and transcription| title | Genomic distancing in yeast: the control of conflicts between DNA-associated machineries and transcription |
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| start_date | 2023/10/27 |
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| schedule | 11h |
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| online | no |
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| location_info | Salle de conférence | 4R4-RDC |
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| details | CBI séminaire public |
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| summary | Sen1 is a helicase that terminates transcription of non-coding RNAs but can also - independently - remove RNA pol II from sites of conflicts with other DNA-associated processes. We are interested in the role of Sen1 at sites of conflicts of transcription with replication and with RNA pol I and RNA pol III transcription. Sen1 has also been involved in the resolution of R-loops, formed when the presumably nascent RNA associates with the DNA template strand. R-loops have physiological roles, but are also considered as genotoxic. I will present our work on the role of Sen1 in conflicts and will describe a new method, H-CRAC, to detect R-loops genomewide with unprecedented resolution and sensitivity. I will present unpublished data on the distribution of H-CRAC signals supporting a new model for the formation of R-loops in yeast. |
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| responsibles | Contact CBI |
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Workflow history| from state (1) | to state | comment | date |
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| submitted | published | | 2023/10/18 13:24 UTC |
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