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Activity of Layer 1 NDNF interneurons (and L2/3 pyramidal neurons) across vigilance states and their role in regulating cortical sleep oscillations| title | Activity of Layer 1 NDNF interneurons (and L2/3 pyramidal neurons) across vigilance states and their role in regulating cortical sleep oscillations |
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| start_date | 2026/10/26 |
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| schedule | 11h |
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| online | no |
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| location_info | salle de conférence |
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| summary | Layer 1 is a hub for synaptic integration in the cortical column. Indeed, it contains the apical dendrites of a large proportion of layer 2/3 and layer 5/6 pyramidal neurons and a dense network of axons from local neurons but also from distant areas. Synaptic integration is locally modulated by GABAergic neurons and in particular NDNF-expressing (L1 NDNF) interneurons. These interneurons can powerfully hyperpolarise dendritic tufts through GABAB receptor activation, controlling the excitability of the cortical column and information integration. During Non-Rapid Eye Movement (NREM) sleep, the cortical column alternates between a silent, hyperpolarised, Down-state and an active, depolarised, Up-state, which are regulated by the thalamocortical loop. We showed that L1 NDNF interneurons play a central role in locally promoting Down-states. Moreover, thalamocortical spindles (12-16 Hz oscillations) and hippocampal ripples (150-250 Hz) are also recorded during NREM sleep and their coordination is crucial for memory consolidation. We tested whether L1 NDNF interneurons could play a role in coordinating these oscillations. Using calcium imaging in freely moving mice, we identified three populations of L1 NDNF neurons that show preferential activity for specific vigilance states, which we did not observe in L2/3 pyramidal neurons. These three populations tend to form separate cell assemblies and show differential activity relative to sleep oscillations in particular relative to ripples. This suggests that they could be differentially involved in gating hippocampal information. We are now asking whether L1 NDNF interneurons could gate hippocampal information in the retrosplenial cortex during sleep following performing a spatial navigation task. |
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| responsibles | Burban |
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Workflow history| from state (1) | to state | comment | date |
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| submitted | published | | 2026/10/20 07:01 UTC |
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